Apart from the remarks already made I would like to add the following:
FFPE slides are strictly pre-fixed (first fixed as a block, then cut
and stained) and cryostat sections from a fresh frozen tissue block
are post-fixed (first cut, then fixed and stained). We have seen a
great difference between post-fixation and pre-fixation with IHC of
small proteins like cytokines. It appeared that the cytokine leaks
away in the fixative in the post-fixation situation, whereas
pre-fixation works fine. I will send the paper we wrote as attachment
to your private mail. This year a better overview on this matter will
be published in Biotechnic & Histochem.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
Date: Tue, 6 Mar 2007 13:22:05 -0500
From: "Kalleberg, Kristopher"
Subject: [Histonet] paraffin vs. frozen
If anyone could give some insight as to what the differences are
frozen sections and paraffin sections when performing IHC I would
greatly appreciate it. I am running a study and the results for a few
of the markers I am not to happy with and am contemplating changing
to frozen sections for upcoming studies. Is there any rhyme or reason
as to why antibodies work better with frozens sections or paraffin
sections? Any help will of course be greatly appreciated.
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Trumbull, CT 06611
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