Hi,

I'm looking to carry out double immunostaining on formalin fixed and 
paraffin blocked mouse kidney sections. I need to stain for proliferating 
macrophage therefore need to use F4/80 marker for macrophage and PCNA for 
proliferating cells on the same sections. At the moment when I carry out 
single staining, I use trypsin antigen retrieval for F4/80 and citrate 
antigen retrieval for PCNA. If you have any suggestions around how I could 
optimise both stainings on the same sections, I would much appreciate it.

Regards,
Sony.

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