Try to mordant the stain with 5% Phosphormolybdenacid or
Phosphortungstenacid for 10 min before Anilinblue (3-5min). 
The PMA should block Cytoplasma staining of Anilinblue. If it isn't
sufficient prolong the incubation time.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jo-Ann
Bader, Ms.
Gesendet: Montag, 05. März 2007 15:02
An: Histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Collagen Staining

Good Morning Everyone,

I am looking for a stain for Collagen without a background stain.  I have
tried the Aniline blue from the Masson's Trichrome with unsatisfactory
results.  

Jo-Ann Bader


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet