Re: [Histonet] tryptase IHC

From:Patti Loykasek

We are using a different clone - clone G3 from Chemicon. In our validation
we did see some cross reactivity with basophils. It was positive on all
cases of mast cell disease we tested & negative on other neoplasms &
hematopoietic cell types.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

>  Hello!
>  We  are  having some trouble with our IHC stain for mast cell tryptase
>  (Dako  AA1  clone).  We seem to get granular intracytoplasmic staining
>  in  several  cells that are probably not mast cells but histiocytes or
>  neoplastic  plasma  cells.   I know that the antibody is very specific
>  for  mast  cells and should not label anything else.  So, does anybody
>  have any ideas?  Could it be the retrieval?  Or the secondary?  We use
>  EnVision so it should not be a problem.
>  Does anybody have any suggestions or has encounter a similar problem?
>  Maria Forzan
>  Finn Pathologists
>  44 (0)1379 854180
>  "Pebbles are inevitable"
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