Re: [Histonet] microwave processing???

From:Rene J Buesa

  1-If your intent is to buy a tissue processor that uses MW technology, the vendor for sure will provide you with the required protocols.
  2-If you are just buying a laboratory designed MW oven to process tissue with personal intervention in each step, that is something else and you will need to use help from others that have done that.
  If you are going to follow "Rute 1" you have nothing to worry about, the technology works.
  If you are going to follow "Rute 2" besides the imput you may receive from colleagues, I would suggest to you to try to obtain the following 2 books:
  "The Microwave Tool Book" by Login and Dvorak. They tell you about generalities (that you need to kow) and about fixation (it does not go farther than that), and
  "Microwaves for the Art of Microscopy" by Kok and Boon. This is more specialized and can guide you through the whole process including staining.
  Following "Rute 2" will be venturous and difficult and you will need all the help you can get. There are also many discussions on the subject in Histonet archives and many publications.
  Hope this will help you!
  René J. wrote:
  Hello Fellow Histonetters:

We are currently planning to bring microwave technology into our lab. The 
problem being no one has any experience with processing tissue with microwave. I 
was hoping someone could guide me to some information about this techniques 
and to answer other question as we go along. Any and all help would be 
greatly appreciated.


Jim Bradford
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