Re: [Histonet] Mayer's Hematoxylin
Thanks for the mention of StainsFile. The hemalum given is still there, but
under the author's name. It is Krutsay's Hemalum. I had it in there twice,
so removed the version without the identification.
----- Original Message -----
From: "Maxim Peshkov"
Sent: Thursday, March 16, 2006 7:15 AM
Subject: Re: [Histonet] Mayer's Hematoxylin
> Try follows:
> "Mayer - HCl
> Alum Hematoxylin
> Hematoxylin 1g
> Aluminum ammonium sulphate 50g
> Distilled water 1L
> Sodium iodate 0.2g
> Hydrochloric acid 1 mL
> Compounding procedure
> Dissolve the alum and hematoxylin in the water.
> Add the sodium iodate, and bring to the boil for
> a few minutes.
> Cool to room temperature and filter.
> Add the concentrated hydrochloric acid.
> Notes and comments
> The solution may be used immediately.
> It is stable for a few months only.
> Calcium deposits are removed.
> Staining time
> 5 - 10 minutes.
> Staining characteristics
> A progressive formulation.
> Gives very precise nuclear staining.
> No other structures are stained.
> Suggested use
> Counterstaining nuclei.
> Precisely nuclear H&E."
> For more detail address to Bryan Llewellin.
> (More thank You, Bryan!)
> This info I was found in www.Stainsfile.info on
> 2003 year. Now his no there.
> This recipe work very well for me over 1 year.
> In this solution I add 10 drops of Tween 20 per 1
> L. This prevented form the surface film.
> Maxim Peshkov.
> maito: Maxim_71@mail.ru
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