Re: [Histonet] Mayer's Hematoxylin

From:Maxim Peshkov

Try follows:

"Mayer - HCl
Alum Hematoxylin
Hematoxylin 1g
Aluminum ammonium sulphate 50g
Distilled water 1L
Sodium iodate 0.2g
Hydrochloric acid 1 mL

Compounding procedure
Dissolve the alum and hematoxylin in the water.
Add the sodium iodate, and bring to the boil for
a few minutes.
Cool to room temperature and filter.
Add the concentrated hydrochloric acid. 

Notes and comments
The solution may be used immediately.
It is stable for a few months only.
Calcium deposits are removed. 

Staining time
5 - 10 minutes. 

Staining characteristics
A progressive formulation.
Gives very precise nuclear staining.
No other structures are stained. 

Suggested use
Counterstaining nuclei.
Precisely nuclear H&E."

For more detail address to Bryan Llewellin.
(More thank You, Bryan!)
This info I was found in on
2003 year. Now his no there.
This recipe work very well for me over 1 year.
In this solution I add 10 drops of Tween 20 per 1
L. This prevented form the surface film.

Maxim Peshkov.

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