Re: [Histonet] Mayer's Hematoxylin
Try follows:
"Mayer - HCl
Alum Hematoxylin
Hematoxylin 1g
Aluminum ammonium sulphate 50g
Distilled water 1L
Sodium iodate 0.2g
Hydrochloric acid 1 mL
Compounding procedure
Dissolve the alum and hematoxylin in the water.
Add the sodium iodate, and bring to the boil for
a few minutes.
Cool to room temperature and filter.
Add the concentrated hydrochloric acid.
Notes and comments
The solution may be used immediately.
It is stable for a few months only.
Calcium deposits are removed.
Staining time
5 - 10 minutes.
Staining characteristics
A progressive formulation.
Gives very precise nuclear staining.
No other structures are stained.
Suggested use
Counterstaining nuclei.
Precisely nuclear H&E."
For more detail address to Bryan Llewellin.
(More thank You, Bryan!)
This info I was found in www.Stainsfile.info on
2003 year. Now his no there.
This recipe work very well for me over 1 year.
In this solution I add 10 drops of Tween 20 per 1
L. This prevented form the surface film.
Sincerely,
Maxim Peshkov.
Taganrog.
Russia.
maito: Maxim_71@mail.ru
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