Re: [Histonet] GMA and Humidity?
The GMA will be affected by humidity to the point that softened plastic is
sometimes a problem to section. Just place your blocks in a dessicator or
some type of plastic box with a lid (rubbermaid, etc) containing a
dessicant i.e. anhydrous calcium chloride inside a mesh bag (recycled knee
high nylons are good!) contains this stuff. Let blocks sit overnight or
several hours to keep plastic from water exposure.
Drierite is a good dessicant , comes in bulk, and is anhydrous calcium
chloride with a blue indicator so you know when it is exhausted - it turns
pinkish. 16 mesh silica gel with indicator from Fisher work is another
and also good and doesn't exfoliate fine CaCL2 dust.
09:14 AM 3/28/2006, you wrote:
>I am new to the GMA process - we just started method development about a
>month ago. Thank you for all of your replies regarding H&E staining. I
>haven't had the opportunity to try any of them yet, but it's on my list
>for this week.
>My new question is - I have been reading a lot regarding GMA and
>humidity. We are currently using the JB-4 kit, and have not had any
>problems yet; however, it's still chilly here and the humidity hasn't
>kicked in yet. I live in southern Indiana, the heart of humidity country
>in the summer and fall!! And our building facilitators sometime have
>trouble controlling the humidity inside when it's 90 degrees with 95%
>humidity outside. What type of problems should I expect, and is there
>something that gives less humidity problems than JB-4?
>Bristol-Myers Squibb, PRI
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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