Re: [Histonet] DAB in tablet form

From:"Andrea T. Hooper"

I too am in academic labs so relate to the cost 
concern; however, I strongly recommend DAB in 
liquid kit form - either DAB+ from DAKO or from 
Zymed. They are liquid ready to use concentrates 
diluted into buffer either supplied with the kit 
or you provide yourself.

I have used both for years and years now with 
excellent results. Worth the extra bucks (about 
80-100 dollars for 110 mls which is actually 
quite a lot) for consistent results time after 
time after time - without the hazards of working 
with powder or solids.

Good luck!
Andrea


>Dear Histos,
>
>Due to the hazards of working with 3,3 diaminobenzidine tetrahydrochloride
>(DAB) for muscle enzyme staining, I am considering purchasing DAB as either
>5mg, or 10mg tablets.  Some companies have them dissolve in water and others
>in a buffer solution. 
>
>I am looking for input from anyone who has used these tablets.  Do you like
>them, do they work well, staining results, which company, etc....  I know
>that you can also get DAB in sealed separate vials, but it is much more
>expensive and we are in an academic research setting where cost is a
>consideration.
>
>I am particularly interested in using them in the Cytochrome Oxidase stain.
>I have tried many procedures for this stain and my Dr. is not all that
>pleased with the results.  If anyone has a good procedure for this stain, I
>would be grateful if you would send me a copy.
>
>I am revamping this lab and have had a lot of questions for the histonet that
>you have been very helpful in addressing.
>
>Thanks for all the suggestions in advance.
>
>
>Joyce Meints
>Histologist
>
>University of Minnesota
>Paul and Sheila Wellstone Muscular Dystrophy Center
>MMC 206
>420 Washington Ave. SE
>Minneapolis, MN 55455-0392
>
>e-mail:    meint002@umn.edu
>Fax: 612-625-8488
>lab phone: 612-626-4703

-- 

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