I have used mouse anti-rat from BD at .1ug/ml on frozen rat sections 
with Dako's LSAB2 rat kit (standard protocol) with good results.

Patsy Ruegg wrote:

>I was wondering what is being used for CD8 labeling of rat tissue?  I assume
>that it still has to be done on frozen tissue?  I have done a lot of rat
>anti-cd8 on mouse tissue using BD antibody but now I need to do it on rat
>tissue.
>Thanks,
>Patsy
> 
>Patsy Ruegg, HT(ASCP)QIHC
>IHCtech, LLC
>Fitzsimmons BioScience Park
>12635 Montview Blvd. Suite 216
>Aurora, CO 80010
>P-720-859-4060
>F-720-859-4110
>wk email pruegg@ihctech.net
>web site www.ihctech.net  
> 
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