Re: [Histonet] CD10

From:"Richard Cartun"

A 1:5 (or 1:10) dilution of an antibody doesn't sound very practical in terms of cost per slide.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Rene J Buesa  03/29/06 03:39PM >>>
  I used CD10 from Novocastra at a dilution of 1:5 with pH6 HIER
  I know of a lab in the UK that dilutes it 1:10 (and seem to work OK, I have seen their slides).
  Hope this will help!
  René J.

Theresa Rohr  wrote:

I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval.

Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. 

Does anyone out there agree/disagree??? 
At what dilution???? 

What I already researched looks like this is not a cheap antibody in terms of dillution. 

I would appreciate any help.

Thanks so much,
Theresa Rohr, Nyack Hospital 

Theresa Rohr, BA, HT(ASCP)
Section Head, Histology
Nyack Hospital
160 North Midland Avenue
Nyack, New York 10960
phone 845-348-2276
fax 845-348-8430

Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. 

Histonet mailing list 

Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates.
Histonet mailing list


Histonet mailing list

<< Previous Message | Next Message >>