Re: [Histonet] CD10

From:Patti Loykasek

Hi Theresa. We use the Novocastro antibody at 1:100 with a heat citrate
pretreatment, and Env+ detection done on the Dako Autostainer. For this
antibody our pretreatment is done in a microwave pressure cooker, perhaps
that is why our titer is more dilute than some others have reported. It is a
nice antibody, but not cheap.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

> Hello,
> I was wondering if anyone has any suggestions regarding CD10. I have the Dako
> Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval.
> Since Dako only has an antibody for use on Frozen sections, I am searching for
> one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a
> great antibody. 
> Does anyone out there agree/disagree???
> At what dilution????
> What I already researched looks like this is not a cheap antibody in terms of
> dillution. 
> I would appreciate any help.
> Thanks so much,
> Theresa Rohr, Nyack Hospital
> Theresa Rohr, BA, HT(ASCP)
> Section Head, Histology
> Nyack Hospital
> 160 North Midland Avenue
> Nyack, New York 10960
> phone 845-348-2276
> fax 845-348-8430
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