Re: [Histonet] CD10

From:"Dana Settembre"

Hello Theresa,
I use CD10 from NovoCastra at a 1:40 dilution.  I use it with Trilogy
in the steamer from Cell Marque as retrieval.
I also use Env+ and the Dako Autostainer.
Good Luck

Dana Settembre
University Hospital - UMDNJ
Newark, NJ

>>> Theresa Rohr  03/29/06 2:39 PM >>>

I was wondering if anyone has any suggestions regarding CD10. I have
the Dako Autostainer and use the Envision+ system and a Steamer for
Antigen Retrieval.

Since Dako only has an antibody for use on Frozen sections, I am
searching for one for FFPE tissue. My Dako Rep actually suggested
Novocastro as having a great antibody. 

Does anyone out there agree/disagree??? 
At what dilution???? 

What I already researched looks like this is not a cheap antibody in
terms of dillution. 

I would appreciate any help.

Thanks so much,
Theresa Rohr, Nyack Hospital 

Theresa Rohr, BA, HT(ASCP)
Section Head, Histology
Nyack Hospital
160 North Midland Avenue
Nyack, New York 10960
phone 845-348-2276
fax 845-348-8430

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