RE: [Histonet] (no subject)

From:Kemlo Rogerson

While I beg to differ;

Gram Weigert's iodine is in fact 1g of iodine with 2g of potassium iodide in
100 mls dist water and is the beast in question.

Gram's iodine is 1g of iodine with 2g of potassium iodide in 300 mls dist
water and is therefore more dilute than 'meint200' wanted.

Lugol's "rubefacient solution" is 1g of iodine with 2g of potassium iodide
in 12 mls of dist water; as you say the stronger solution but I don't 'do'

Test: Cowdry's iodine and Langeron's iodine, any idea?

I did stifle the comment about magnetic iodine but I assume 'reagent' iodine
is different over there.

Kemlo Rogerson
Pathology Manager
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-----Original Message-----
From: John A. Kiernan [] 
Sent: Thursday, March 16, 2006 5:13 PM
To: meint002; Histonet
Subject: Re: [Histonet] (no subject)

Could your "iodine" really be iron or magnetic
iron oxide? Magnets don't pick up bits of iodine!
Iodine is insoluble in water but it dissolves
quite quickly in an aqueous solution of potassium

It's good to see that you're correctly calling the
solution Gram's iodine. Many people call it
Lugol's, which is a stronger solution (6% iodine
in 4% KI).
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
meint002 wrote:
> Dear Histos,
> I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium
> 100ml distilled water to start)and I can't get the iodine to go into
> solution.  If I use a stir bar the iodine is attracted to the magnet and
> doesn't come off.  I've also tried heat and that doesn't seem to work
>  All the text books I've looked at say shake until dissolved.  Tried that,
> the iodine just sits in the bottom of the beaker and nothing.
> I don't remember having this problem before in school.  All suggestions
> welcome.  Thanks in advance.
> Joyce Meints
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