We were all taught this back in histo. school......and Sheehan, while
not giving the details as to why, describes the "recipe" much as Pat
wrote. 


Jeanine Bartlett, BS, HT(ASCP)
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Koelling, Ray
Sent: Thursday, March 16, 2006 1:45 PM
To: Greer, Patricia; meint002; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

For those struggling to make up Gram's Iodine, you should look up what a
chaotropic agent is (Potassium Iodide is one)and why then mixing things
just as Pat wrote makes this so easy.

Ray

Raymond Koelling
Research Scientist
Pathology
Amgen Corporation
Seattle, WA

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Greer,
Patricia
Sent: Thursday, March 16, 2006 10:23 AM
To: meint002; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)


Joyce,

In order to get the iodine into solution, first dissolve the 2g
potassium iodide in  4-5 ml of water and dissolve the 1g of iodine in
this.  Dilute to 100 ml.  
These instructions are not often printed with the formula for making
Gram's iodine and I remember how frustrating it was the first time I
tried to make it up.  Then I read the directions in Culling's "Handbook
of Histopathological and Histochemical Techniques"

Hope this helps.

Pat Greer
Infectious Disease Pathology Activity
Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA
30333

404-639-2811


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of meint002
Sent: Thursday, March 16, 2006 11:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Dear Histos,

I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium
iodide, 100ml distilled water to start)and I can't get the iodine to go
into solution.  If I use a stir bar the iodine is attracted to the
magnet and doesn't come off.  I've also tried heat and that doesn't seem
to work either.
 All the text books I've looked at say shake until dissolved.  Tried
that, the iodine just sits in the bottom of the beaker and nothing.

I don't remember having this problem before in school.  All suggestions
are welcome.  Thanks in advance.

Joyce Meints



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