RE: [Histonet] bone IHC

From:"Elizabeth Chlipala"


We use proteinase K digestion for Collagen I on formic acid decalcifed
bone sections, we get our Collagen I from Biogenesis.  For Collagen II
we use no digestion and get our antibody from MD Biosciences.  Its pricy
but we find it works the best for us.  I have tried the lab vision
antibody and had some problems with it.  I like the MD biosciences
antibody because it's a rabbit polycolonal and a lot of our samples are
generated in mouse, but we also run it on rat, sheep, and goat


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Tamara
Sent: Thursday, March 16, 2006 10:42 AM
Subject: [Histonet] bone IHC

Anyone with experience in collagen I staining on decalcified bone
paraffin sections? Would this require more digestion than say collagen
II on cartilage. any other differences? Collagen II is working perfectly
but collagen I refuses to work.  Thanks. Tamara

Tamara Franz-Odendaal
Biology Dept., Dalhousie University
Halifax, NS, B3H 4J1
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