RE: [Histonet] Frozen embedding of lungs

From:Kemlo Rogerson

When I was a pup I made a little 'lung tank'; you construct a piece of
apparatus with a header tank at height to produce 1 atmosphere of pressure
(can't remember the calculation but I think it was a metre), fill header
tank with 10% buffered formalin and attached to trachea with a pipe. The
pressure will inflate the lung but formalin will drip out; I put a reservoir
beneath and then pumped the formalin back up to the header.

I never used a respirator or anything and I guess that's why people say I
look young for me age; use a respirator!!! I used it a lot for miner's black
lung in an area that had coal mining for pneocony.... pneumoconiu..... um...
Miner's Black lung! 

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194

"Following the line of least resistance makes both men and rivers crooked"


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-----Original Message-----
From: Kim O'Sullivan [] 
Sent: Thursday, March 30, 2006 1:47 AM
Subject: [Histonet] Frozen embedding of lungs

Hi all,

Does any one out there have a successful method for inflating mouse lungs to
freeze. We currently freeze them unfixed in OCT, but of course the
morphology is shocking as the lungs have collapsed. Is there a method that
preserves the morphololgy without collapsing or imploding the lungs.

Any advice will be appreciated

Kim O'Sullivan
Centre for Inflammatory Diseases
Department of Medicine
Monash University

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