RE: [Histonet] Fixation sensitive IHC markers

From:Luis Chiriboga

you might want to take a look at:
"Assessment of antigen damage in immunohistochemistry. the Vimentin internal
control" by Hector Battifora. American Journal of Clinical Pathology
November 1991 96(5) 669.
Hope it helps

Luis Chiriboga Ph.D.
NYU Cancer Institute and
Bellevue Hospital Center
New York University School Of Medicine
Department Of Pathology 4W27
462 First Avenue
New York, N.Y. 10016
W(212) 562-4667.
F(212) 263-2041

-----Original Message-----
[]On Behalf Of Thomas
Sent: Wednesday, March 22, 2006 2:41 PM
Subject: [Histonet] Fixation sensitive IHC markers

Dear Histonetters,

Can anyone suggest an antibody that I could use on human FFPE samples that
shows relative degrees of reactivity, based on variable factors such as
fixation time or tissue autolysis?  I would like to assess the reactivity
of commercially available tissue bank samples.

Any suggestions would be appreciated.

Tom Crowell
Biogen Idec
Cambridge, MA
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