RE: [Histonet] Fixation sensitive IHC markers

From:Luis Chiriboga

you might want to take a look at:
"Assessment of antigen damage in immunohistochemistry. the Vimentin internal
control" by Hector Battifora. American Journal of Clinical Pathology
November 1991 96(5) 669.
Hope it helps
Luis

____________________________________
Luis Chiriboga Ph.D.
NYU Cancer Institute and
Bellevue Hospital Center
New York University School Of Medicine
Department Of Pathology 4W27
462 First Avenue
New York, N.Y. 10016
W(212) 562-4667.
F(212) 263-2041



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Thomas
Crowell
Sent: Wednesday, March 22, 2006 2:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fixation sensitive IHC markers


Dear Histonetters,

Can anyone suggest an antibody that I could use on human FFPE samples that
shows relative degrees of reactivity, based on variable factors such as
fixation time or tissue autolysis?  I would like to assess the reactivity
of commercially available tissue bank samples.

Any suggestions would be appreciated.

Tom Crowell
Biogen Idec
Cambridge, MA
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