RE: [Histonet] CD10

From:"Tony Henwood"

We use Novocastra's NCL-CD10-270 1/100 dilution with EDTA retrieval (20min) on the Bond Max 


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: [] On Behalf Of Rene J Buesa
Sent: Thursday, 30 March 2006 7:40 AM
To: Theresa Rohr;
Subject: Re: [Histonet] CD10

  I used CD10 from Novocastra at a dilution of 1:5 with pH6 HIER
  I know of a lab in the UK that dilutes it 1:10 (and seem to work OK, I have seen their slides).
  Hope this will help!
  René J.

Theresa Rohr  wrote:

I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval.

Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. 

Does anyone out there agree/disagree??? 
At what dilution???? 

What I already researched looks like this is not a cheap antibody in terms of dillution. 

I would appreciate any help.

Thanks so much,
Theresa Rohr, Nyack Hospital

Theresa Rohr, BA, HT(ASCP)
Section Head, Histology
Nyack Hospital
160 North Midland Avenue
Nyack, New York 10960
phone 845-348-2276
fax 845-348-8430

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