RE: [Histonet] CAP Immuno Survey Slides

From:"Dawson, Glen"


I've talked to the CAP about this issue and they said that they would "take it under advisement".  Just goes to show you that those in charge of sending out these CAP surveys for IHC have no experience in the actual staining of IHC slides.

Also, the labels they put on are heavy duty and the adhesive they leave behind is NOT condusive to automated coverslipping (with the Sakura Glass coverslipper).  They tend to fall off the suction cup and get broken by the conveyor.  I would strongly suggest coverslipping these slides by hand until the CAP decides to stop this craziness.

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
[]On Behalf Of Dana
Sent: Thursday, March 30, 2006 12:40 PM
To: JUAN GUTIERREZ; Richard Cartun; Histonet (E-mail); Laura Jones
Subject: RE: [Histonet] CAP Immuno Survey Slides

Please do not send us slides with labels.
They were really difficult to work with.

Dana Settembre
University Hospital - UMDNJ
Newark, NJ

>>> Richard Cartun  03/30/06 12:59 PM >>>
I agree.  I will forward this to a member of the CAP Cell Markers


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "GUTIERREZ, JUAN"  03/30/06
12:44PM >>>
We pulled the labels off.  What a pain in the ....  To: CAP,  please
don't do that again.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor

My opinions are my own and do not reflect those of my employer.  Long
live free speech!

-----Original Message-----
[] On Behalf Of Jones,
Sent: Thursday, March 30, 2006 11:05 AM
To: Histonet (E-mail)
Subject: [Histonet] CAP Immuno Survey Slides

Hello Histoland.  We were wondering if anyone else who may have
in the recent CAP survey had experienced any problems with the labels
slides.  We performed some of the survey by hand (our machine is
using the Shandon Sequenza coverplates, and it seemed like the label
adhesive was blocking the reagents from passing onto the slide.  We
repeated some of the slides on a demo machine, after verifying our
antibodies, and even then it seemed like that adhesive was interfering
the reagents.  We also lost any info we had written on the slide
even with the impervious markers, during automated processing.  

We didn't remember our survey slides ever having labels before, and
curious if anyone else had encountered this.  Thanks in advance!  

The Histochicks at Sharon Regional, Sharon, PA

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