RE: [Histonet] CAP Guidelines for Microwaves

From:Rene J Buesa

Greg;
  That is essentially my point. In the same way that you never would try to enter the Monaco race with a small FIAT 600 (but with a Porsche) you should never try to do things with a household MW oven that will require a lab designed one.
  Things I did (for years) with my 2 MW ovens: dry slides, all sorts of boosts to special stains, heating the citrate buffer before the HIER procedure and things of that sort.
  Never to process tissue (although I had to confess some "indiscretionts in that realm").
  Regarding durability, my MW was used for more than 10 years continuously.
  Regarding reproducibility: it CAN be obtained as long as you calibrate it and know their capabilities [I even published a paper on that topic].
  Now to the original question about CAP regulations and household MW ovens: the answre and approach from their point of view is the same: "do NOT used them" [for that purpose].
  René J.

"Luck, Greg D."  wrote:
  Oh Why Not,

I've taken the position (that from the CAP comment section of this question)
that use of a non commercial grade microwave is still safe and usable
provided you limit its use to heating of reagents such as PBS, agar, and
tris or citrate buffers.

Greg Luck
Anatomic Pathology Supervisor
Deaconess Med Center / EHS
800 W. 5th Ave
Spokane, WA 99204
Phone 509.473.7394
Fax 509.473.7133
luckg@empirehealth.org

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] 
Sent: Friday, March 24, 2006 4:17 AM
To: Angela Bitting; histonet@lists.utsouthwestern.edu;
jnocito@pathreflab.com; jnocito@satx.rr.com; HParker@Skaggs.Net;
rjbuesa@yahoo.com
Subject: RE: [Histonet] CAP Guidelines for Microwaves

Heating Bouin's is an issue, I would think. 


Jeanine Bartlett, BS, HT(ASCP)
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Friday, March 24, 2006 7:09 AM
To: histonet@lists.utsouthwestern.edu; jnocito@pathreflab.com;
jnocito@satx.rr.com; HParker@Skaggs.Net; rjbuesa@yahoo.com
Subject: Re: [Histonet] CAP Guidelines for Microwaves

Are any of the chemicals we heat in our microwaves toxic? I don't think most
special stains solutions fall in that catagory. That's the first question to
answer before getting to wrapped up in this ventilation issue? 

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916

>>> "Joe Nocito" 3/23/2006 7:42 PM >>>

but what about using a table top fume absorber with charcoal filters? CAP
writes these questions so ambiguously that it becomes a joke. This is the
main reason why I have a negative attitude towards CAP.

Joe
----- Original Message -----
From: "Rene J Buesa" 
To: "Joe Nocito" ; "'Parker, Helayne'" 
; 
Sent: Thursday, March 23, 2006 4:19 PM
Subject: RE: [Histonet] CAP Guidelines for Microwaves


> Joe:
> It is not external ventilation to the MW oven what CAP wants, is a vent 
> connected from the microwave to an outlet and there is not a single 
> household MW oven with that characteristec so, for all practical purposes 
> the mensage keeps being: don't use them (the household appliance) for 
> histology work, specially if tissue processing is the objective of the 
> use.
> René J.
>
> Joe Nocito wrote:
> I don't believe CAP is saying not to use them. They are saying use them
> safely with ventilation and make sure they don't leak radiation.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J 
> Buesa
> Sent: Thursday, March 23, 2006 11:35 AM
> To: Parker, Helayne; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] CAP Guidelines for Microwaves
>
> Helayne:
> In short, the message from CAP is: "do not use them!"
> Unfortunate though!
> René J.
>
> "Parker, Helayne" wrote:
> I was wondering if anyone new what CAP require of us if we have a common
> household microwave that we use for special stains.
>
> Helayne Parker,HT (ASCP)
> Histology Section Head
> Skaggs Community Health Center
> Branson, Missouri
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Blab-away for as little as 1˘/min. Make PC-to-Phone Calls using Yahoo!
> Messenger with Voice.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
> ---------------------------------
> Blab-away for as little as 1˘/min. Make PC-to-Phone Calls using Yahoo! 
> Messenger with Voice.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> -- 
> No virus found in this incoming message.
> Checked by AVG Free Edition.
> Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006
>
> 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




		
---------------------------------
New Yahoo! Messenger with Voice. Call regular phones from your PC and save big.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>