[Histonet] tryptase IHC
We are having some trouble with our IHC stain for mast cell tryptase
(Dako AA1 clone). We seem to get granular intracytoplasmic staining
in several cells that are probably not mast cells but histiocytes or
neoplastic plasma cells. I know that the antibody is very specific
for mast cells and should not label anything else. So, does anybody
have any ideas? Could it be the retrieval? Or the secondary? We use
EnVision so it should not be a problem.
Does anybody have any suggestions or has encounter a similar problem?
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