[Histonet] paraffin tissue embedding and cutting

From:"karen Cai"

Hello there,
I have some questions regarding embedding and cutting paraffin tissue
block:
 
1.	After embedding, I found there are a lot of bubbles inside the
paraffin tissue block (I put the mold at RT). Sometimes it has,
sometimes not.  I donít know why. How to avoid this?
2.	 Sometimes I found if I put the paraffin tissue sections into
the 37C water bath, the paraffin will be diffused very quickly. Also the
color of the whole paraffin tissue block look strange: white, not clear
white. I am jus wondering whether the method of embedding  tissue is
wrong, or  something else?
 
Any kind of help will be very appreciated,
 
Thanks in advance,
 
Best,
Karen
 
 
 

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