[Histonet] dual immunofluorescence

From:"Jurcisek, Joseph"

I would like to label some frozen sections with 2-3 antibodies directed
against different antigenic sites.  I would like to use fluorescence to
identify these Abs. What is the best way to accomplish this.  I have
seen protocols where both primary Abs are mixed in a cocktail as well as
protocols where the primary, secondary, and tertiary (if warranted)is
completed for one antigen and then the process repeated for the second.
I have run both of the Abs singly on similar tissue with decent results
but now I would like to label the same tissue with both.  Any help is
greatly appreciated.


Joseph A. Jurcisek
Senior Research Associate
Center for Microbial Pathogenesis
Columbus Children's Research Institute
700 Children's Dr.
Columbus, OH 43205-2696
(614) 355-3521
fax (614) 722-2818

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