[Histonet] TEM and oysters

From:Anne-Sophie MARTINEZ

Hi everybody. I have 2 questions concerning TEM.
(1) I have fixed gonads of adult oysters for TEM with glutaraldehyde, 
post-fixed with osmium and done the embedding in Epon. Is there any way 
I can you do immunocytochemistry with antibodies on these samples?
(2) How can I decalcify the shelves of larvae and juveniles oysters for 
TEM without damaging the tissue?
Could anyone help me please? Thanks for your suggestions.

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