[Histonet] Staining Glycol Methacrylate

From:Leslie D Duncan


I am experimenting with glycol methacrylate embedded rodent testes.  I 
am looking for a good H&E staining protocol to use that will give 
results comparable to paraffin-embedded tissue (per request of my 
management).  We have dehydrated on the tissue processor to 100% 
ethanol, ilfiltrated and embedded with JB-4, and sectioned at 1-2 
microns.  The sections are placed on Poly L-lysine slides.  Any 
protocols, ideas, and/or suggestions will be greatly appreciated!

Thank you,
Leslie Duncan
Bristol-Myers Squibb, PRI

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