[Histonet] Re: message 16 tunel problems
| From: | "Carlos G. Perez-Garcia" |
Hi,
I suggested you to boiling samples in citrate buffer at ph 6 it
can be helpful to your problems of background, i work in brain and is
very useful for tunel, mostly in paraffin sections.
best
c
Carlos G. Perez-Garcia, Ph.D.
Molecular Neurobiology Lab (MNL-O)
The Salk Institute
10010 North Torrey Pines Road
92037 La Jolla, CA
USA
cpgarcia@salk.edu
Fax: 858 558 6207
On Mar 17, 2006, at 10:08 AM, histonet-
request@lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Somewhat rhetorical question regarding antibody incubation
> time (Jackie M O'Connor)
> 2. Re: Cd8 on rat tissue (Susan Q Wells)
> 3. Commercial micro waves (Doug Geddes)
> 4. RE: Histonet Digest, Vol 28, Issue 27 (Orr, Rebecca)
> 5. double stain (Orr, Rebecca)
> 6. Re: Commercial micro waves (Phil McArdle)
> 7. Training Med Techs (Orr, Rebecca)
> 8. Re: Somewhat rhetorical question regarding antibody
> incubation time (Rene J Buesa)
> 9. New NY regulations (KDwyer3322@aol.com)
> 10. Region IX Education Day (Mark Elliott)
> 11. RE: Sudan Black Staining (Kellar, Eric C)
> 12. clarifying the MT -HT training (Orr, Rebecca)
> 13. RE: Gram's iodine mystery solved (Smith, Allen)
> 14. An aside on viewing kidney biopsies Re: [Histonet]
> microscopes (Gayle Callis)
> 15. Re: New NY regulations (Judith LaDuc)
> 16. immunohistochemistry and TUNEL background staining (Jacqui
> Detmar)
> 17. Re: Sudan Black Staining (John A. Kiernan)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 17 Mar 2006 06:51:56 -0600
> From: "Jackie M O'Connor"
> Subject: [Histonet] Somewhat rhetorical question regarding antibody
> incubation time
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset="us-ascii"
>
> If you have an antibody that the manufacturer recommends overnight
> incubation at 4C at 1:1200 dilution -
> is there anything to be gained from increasing the concentration
> (1:600)
> and incubating for one hour RT instead?
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 17 Mar 2006 07:53:19 -0500
> From: Susan Q Wells
> Subject: Re: [Histonet] Cd8 on rat tissue
> To: Patsy Ruegg
> Cc: histonet@pathology.swmed.edu
> Message-ID: <441AB13F.30805@bms.com>
> Content-Type: text/plain; format=flowed; charset=us-ascii
>
> I have used mouse anti-rat from BD at .1ug/ml on frozen rat sections
> with Dako's LSAB2 rat kit (standard protocol) with good results.
>
> Patsy Ruegg wrote:
>
>> I was wondering what is being used for CD8 labeling of rat
>> tissue? I assume
>> that it still has to be done on frozen tissue? I have done a lot
>> of rat
>> anti-cd8 on mouse tissue using BD antibody but now I need to do it
>> on rat
>> tissue.
>> Thanks,
>> Patsy
>>
>> Patsy Ruegg, HT(ASCP)QIHC
>> IHCtech, LLC
>> Fitzsimmons BioScience Park
>> 12635 Montview Blvd. Suite 216
>> Aurora, CO 80010
>> P-720-859-4060
>> F-720-859-4110
>> wk email pruegg@ihctech.net
>> web site www.ihctech.net
>>
>>
>> This email is confidential and intended solely for the use of the
>> Person(s)
>> ('the intended recipient') to whom it was addressed. Any views or
>> opinions
>> presented are solely those of the author. It may contain
>> information that is
>> privileged & confidential within the meaning of applicable law.
>> Accordingly
>> any dissemination, distribution, copying, or other use of this
>> message, or
>> any of its contents, by any person other than the intended
>> recipient may
>> constitute a breach of civil or criminal law and is strictly
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>>
>> _______________________________________________
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>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 17 Mar 2006 08:06:53 -0500
> From: "Doug Geddes"
> Subject: [Histonet] Commercial micro waves
> To:
> Message-ID:
> Content-Type: text/plain; charset="US-ASCII"
>
> Would anyone be will to share any information good or bad about
> commercial mircro waves for antigen retrieval, vendors welcomed.
>
>
> Doug Geddes BSc., MLT
> Dept of Pathology
> London Health Sciences Centre
> London, Ontario, Canada
>
> -----------------------------------------
> This information is directed in confidence solely to the person
> named above and may contain confidential and/or privileged
> material. This information may not otherwise be distributed,
> copied or disclosed. If you have received this e-mail in error,
> please notify the sender immediately via a return e-mail and
> destroy original message. Thank you for your cooperation.
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 17 Mar 2006 07:32:28 -0600
> From: "Orr, Rebecca"
> Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 27
> To:
> Message-ID:
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Stacy,
> I wrote a sentence describing the issues of background staining. I
> then
> described what detection kits we use in our lab. We use both
> polymer(Dako/Biocare) and the Ventana I view on the benchmark. I
> also
> added instructions to determine if Endogenous Biotin is evident
> (omitting primary and secondary and just adding the label and
> chromogen
> on one slide, just the chromogen on a second slide...)
> I put something in there about following manufacturer's
> recommendations
> on the data sheets. I addressed protein blocks and endogenous
> peroxidase
> all in the same section and called it "Blocking Reagents".
> I was just inspected in February and the inspectors and I scrutinized
> that regulation especially since it was new. I passed so I'm thinking
> what I wrote was adequate. I'll go ahead and send you the excerpt
> with
> my references in a separate file. Let me know if you can't receive
> files from strangers. I can fax if you'd like
> Have a great weekend
> Becky
>
> Becky Orr CLA,HT(ASCP)
> IHC Lead
> Evanston Northwestern Healthcare
> 847-570-2771
>
>
> -
>
> Message: 6
> Date: Thu, 16 Mar 2006 14:01:46 -0500
> From: "Stacy McLaughlin"
> Subject: [Histonet] Endogenous Biotin and CAP regulations
> To:
> Message-ID:
>
> dickinson.org
>>
>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Would anyone be willing to share how they address this issue,
> regarding
> CAP reg: ANP.22615 "If the laboratory uses an avidin-biotin
> complex
> (ABC) detection system (or a related system such as
> streptavidin-biotin
> or neutravidin-biotin), is there a policy that addresses
> nonspecific
> false positive staining from endogenous biotin?
> We're currently using the Ventana Nexes, with biotin-streptavidin
> detection system.
> Are there specific vendors your recommend for these reagents?
> Your answers are appreciated!
> Stacy
> Stacy McLaughlin HT (ASCP)
> Lead Tech, Histology
> Coolely Dickinson Hospital
>
>
>
> Stacy McLaughlin HT (ASCP)
> Lead Tech, Histology
>
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 17 Mar 2006 08:03:28 -0600
> From: "Orr, Rebecca"
> Subject: [Histonet] double stain
> To:
> Message-ID:
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Rachel,
> I have encountered similar issues in my journey through
> doublestaining.
>
>> From what I know of Triton X, it's mainly a surfactant. I'm not sure
> of the pH. There are solutions on the market that most likely have
> some
> type of surfactant in them, hence all the soapy bubbles in the
> rinsing.
> What solutions have you tried?
>
> What I have been successful with in this type scenario is trying a
> combination of either a lighter (less harsh HEIR) and then either a
> diluted enzyme or less time in the enzyme. Can you alter the
> concentration of the antibodies? One using the triton might need
> higher
> concentration, or more time.
> When you say sequentially, are you taking one ab all the way to the
> chromogen then starting the second one? I would imagine this is your
> protocol, but that would be good to know.
>
> Becky Orr CLA,HT(ASCP)
> IHC Lead
> Evanston Northwestern Healthcare
> 847-570-2771
>
>
>
>
> Message: 28
> Date: Fri, 17 Mar 2006 07:42:38 -0500
> From: "Emerson, Rachael"
> Subject: [Histonet] Double stain ?
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello. I am attempting to do a sequential double stain with 2
> different
> antibodies.
> Unfortunately, one requires using Triton-X 100 for antigen
> retrieval and
> the
> other requires using trypsin.
> I've run trials using several different antigen retrieval methods and
> these
> are the only ones that work.
> I'm afraid that performing 2 different retrieval steps will cause the
> stain
> not to work. I would really appreciate any thoughts or suggestions.
> I am working with paraformaldehyde fixed mouse embryos.
>
>
> Thanks
> Rachael Emerson
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 28, Issue 27
> ****************************************
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 17 Mar 2006 09:10:51 -0500
> From: Phil McArdle
> Subject: Re: [Histonet] Commercial micro waves
> To: Doug Geddes ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <441AC36B.70202@ebsciences.com>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hi:
>
> Check out http://www.ebsstore.com/control/category/~category_id=C
>
> Please feel free to contact me with any questions or issues you may
> have.
>
> Best regards,
>
> Phil McArdle
> Microwave Product Manager
> Energy Beam Sciences, Inc.
> Tel: 800.992.9037 x 341
> Cell: 860.597.6796
> Fax: 860.653.0422
> PMcardle@ebsciences.com
> www.ebsciences.com
> "ADDING BRILLIANCE TO YOUR VISION"
>
> "I hate quotations. Tell me what you know." Ralph Waldo Emerson
>
>
> Doug Geddes wrote:
>> Would anyone be will to share any information good or bad about
>> commercial mircro waves for antigen retrieval, vendors welcomed.
>>
>>
>> Doug Geddes BSc., MLT
>> Dept of Pathology
>> London Health Sciences Centre
>> London, Ontario, Canada
>>
>> -----------------------------------------
>> This information is directed in confidence solely to the person
>> named above and may contain confidential and/or privileged
>> material. This information may not otherwise be distributed,
>> copied or disclosed. If you have received this e-mail in error,
>> please notify the sender immediately via a return e-mail and
>> destroy original message. Thank you for your cooperation.
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 17 Mar 2006 09:04:02 -0600
> From: "Orr, Rebecca"
> Subject: [Histonet] Training Med Techs
> To:
> Cc: "Delk, Linda"
> Message-ID:
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello everyone.
>
> I would appreciate any feedback from those of you who may have had to
> train MT's (ASCP) to work in Histology.
>
> They would be trained as histo techs with the intent to promote them
> into Anatomic Pathology (Histology) management positions.
>
> Candid comments welcome, especially from MT's who now work in
> histology!
>
> To me it would be like trying to train a policeman to be a fireman,
> it's a career, not a job, right?
>
>
>
> We see a HT shortage in the Chicago area, but I am unsure how to
> address
> this.
>
>
>
> Degreed individuals have proven critical thinking skills via a
> traditional education pathway, so I see the advantages, but to ignore
> very capable HT managers with proven management and organizational
> skills via non traditional pathways is becoming an issue with me.
>
> I mean it's not like Non degreed HT's are stooopid or something.
>
>
>
> Thank you
>
> Becky
>
>
>
> Becky Orr CLA,HT(ASCP)
>
> IHC Lead
>
> Evanston Northwestern Healthcare
>
> 847-570-2771
>
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 17 Mar 2006 07:04:50 -0800 (PST)
> From: Rene J Buesa
> Subject: Re: [Histonet] Somewhat rhetorical question regarding
> antibody incubation time
> To: Jackie M O'Connor ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <20060317150450.43863.qmail@web61217.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> If it works it will expedite your TAT and I would certainly
> recommend that approach, just one thing though: you cannot equate a
> 2:1 increased concentration with a 16:1 reduction of incubation
> time + a 6:1 fold increase of temperature.
> You should make several tests to find out the correct new
> dilution factor that will allow you to change the incubation time
> and temperature to what you desire.
> Hope this will help you!
> René J.
>
> Jackie M O'Connor wrote:
> If you have an antibody that the manufacturer recommends overnight
> incubation at 4C at 1:1200 dilution -
> is there anything to be gained from increasing the concentration
> (1:600)
> and incubating for one hour RT instead?
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Yahoo! Travel
> Find great deals to the top 10 hottest destinations!
>
> ------------------------------
>
> Message: 9
> Date: Fri, 17 Mar 2006 10:15:43 EST
> From: KDwyer3322@aol.com
> Subject: [Histonet] New NY regulations
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <22b.87e55fa.314c2c9f@aol.com>
> Content-Type: text/plain; charset="ISO-8859-1"
>
> Histonetters from New York:
> As stated below will the new regulations affect histotechnicians?
> Any help
> would be appreciated.
> Thanks,
> Kathy Dwyer
>
>
>
> The New York State Office of the Professions has posted updated
> information
> on our state licencing of clinical laboratory technologists,
> technicians and
> Cytotechnologists as pasted below the direct link.
>
> http://www.op.nysed.gov/clp-mar06update.htm
>
> March 2006
> The State Education Department, with the assistance of the State
> Board for
> Clinical Laboratory Technology and interested parties are in the
> process of
> developing draft regulations that will be necessary for the
> licensure of the
> professionals as clinical laboratory technologists,
> Cytotechnologists, and clinical
> laboratory technicians. Once these draft regulations are completed,
> they will
> be submitted to all interested parties and published in the State
> Register
> for comment. At the end of this process, regulations will be
> presented for
> consideration and action by the Board of Regents. It is anticipated
> that this
> process will be completed by mid-summer in 2006. Applications
> packets for licensure
> will not be available until the regulations are enacted. Prior to the
> enactment of the regulations, the Department cannot provide any
> specific information
> to individuals regarding licensure under the "Special Provisions" or
> "grandparenting.." As soon as the regulations are enacted, this
> information will be
> available.
>
> As soon as the regulations are enacted by the State Board of
> Regents, the
> Department will publish a Guide to Practice for the professions of
> Clinical
> Laboratory Technology, Clinical Laboratory Technician, and
> Cytotechnology, as well
> as an application packet for these professions. When they are
> available, the
> applications will be published on this website and can be
> downloaded and
> submitted to the Department. Applicants who wish to receive a hard
> copy of the Guide
> to Practice and the Application Packet may submit an e-mail request
> for the
> information now, but should not expect to receive this information
> before the
> summer of 2006. A notice will be put on this website when the
> applications are
> ready to be mailed.
>
> Clinical Laboratory Technicians, Clinical Laboratory Technologists and
> Cytotechnologists who want to request to be placed on a mailing
> list now for a hard
> copy of the application packet and receive it in the summer of 2006
> should
> send an e-mail to OPFORMS@mail.nysed.gov, giving their name and
> address, and
> specify the packet they want by:
>
> Code 92A and profession title: Clinical Laboratory Technologist/
> Clinical
> Laboratory Technician, or
> Code 93A and profession title: Cytotechnology.
>
> http://www.op.nysed.gov/clp-mar06update.htm
> Page last updated: 03/15/2006 12:07:36
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 17 Mar 2006 07:20:07 -0800
> From: "Mark Elliott"
> Subject: [Histonet] Region IX Education Day
> To:
> Message-ID: <441A6327020000D60000464E@mail.mrl.ubc.ca>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi everyone
> For anyone wanting an excuse to visit the wonderful city of
> Montreal, we have the perfect reason-Region IX is hosting an
> Education Day on May 6th, 2006 in this vibrant city. We are having
> 5 speakers on a variety of topics and 13 vendors will be present
> showcasing their products. Registration deadline is April 14th.
> Please visit http://www.nshregionix.org/education.html
> for more information, including registration forms, speaker
> schedule and hotel information.
> Hope to see you there!
> Mark Elliott
> Region IX Education Committee Chair
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Fri, 17 Mar 2006 10:24:16 -0500
> From: "Kellar, Eric C"
> Subject: [Histonet] RE: Sudan Black Staining
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> <6843061CE6B98E4B96590D4F299618F801583BB6@qdcws0117.us.qdx.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> To slow Sudan Black B (a nonionic, hydrophobic dye) 'bleeding' on
> unfixed cryostat sections, try running a duplicate slide and
> compare with this method:
>
> 1. Air dry duplicate slide after staining.
> 2. Clear slide through 3 changes of fresh Xylene or Xylene Substitute.
> 3. Glass coverslip using a permanent mounting media.
>
> The synthetic mountant will eventually dissolve some of the dye-
> lipid complex, but it will give you a longer window of time to
> microscopically examine your slides.
>
>
> Eric C. Kellar
> Histology/Immunohistochemistry
> Quest Diagnostics Inc. Miami
>
>
> Hi,
> I am working on the Sudan Black stain. Do the slides need to be read
> immediately due to bleeding of the stain after approximately 24 hours?
>
> Thanks for any information you can give me about the stain.
> Sharon Willman
>
>
> =========================3D=========================3D====================
> ========
> The contents of this message, together with any attachments, are
> intended only for the use of the person(s) to which they are
> addressed and may contain confidential and/or privileged
> information. Further, any medical information herein is
> confidential and protected by law. It is unlawful for unauthorized
> persons to use, review, copy, disclose, or disseminate confidential
> medical information. If you are not the intended recipient,
> immediately advise the sender and delete this message and any
> attachments. Any distribution, or copying of this message, or any
> attachment, is prohibited.
> =========================3D=========================3D====================
> ========
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Fri, 17 Mar 2006 09:27:50 -0600
> From: "Orr, Rebecca"
> Subject: [Histonet] clarifying the MT -HT training
> To:
> Message-ID:
>
> Content-Type: text/plain; charset="US-ASCII"
>
> In clarification,
>
> I don't think I worded that right. It's a salary compensation
> issue. A
> non degreed HT is expected to take on the same responsibilities as a
> histology supervisor for 10K less than a trained MT crossing over to
> HT.
>
> So to reiterate, it's not so much being eligible to handle to
> management, it's about taking less salary for the same responsibility.
>
>
>
> Thank you all for the feedback this is seems like a warm issue!
>
>
>
>
>
> Becky Orr CLA,HT(ASCP)
>
> IHC Lead
>
> Evanston Northwestern Healthcare
>
> 847-570-2771
>
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Fri, 17 Mar 2006 10:35:48 -0500
> From: "Smith, Allen"
> Subject: RE: [Histonet] Gram's iodine mystery solved
> To: "John Kiernan"
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID:
> <5D2189E74151CC42BEC02906BA8996322B919F@exchsrv01.barrynet.barry.edu>
> Content-Type: text/plain; charset="US-ASCII"
>
> Shockingly, labels are not always correct. I have a jar labelled
> "indigocarmine" whose contents is insoluble in water.
> I once (20 years ago) opened a brand new bottle labelled "acetic
> anhydride" and caught a whiff of isoamyl acetate. The contents
> also boiled
> at the boiling point of isoamyl acetate (4 C higher than acetic
> anhydride),
> and it did not dissolve in hot water.
> I have also borrowed a bottle of bromine from a colleague (25
> years ago
> at another school) and found that it performed brominations fairly
> well, but
> required an elevated temperature to do them. I never did find out
> what the
> contaminant was or how it got there.
>
> Allen A. Smith, Ph.D.
> Professor of Anatomy
> Barry University School of Graduate Medical Sciences
> Podiatric Medicine and Surgery
> Miami Shores, Florida 33161
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> John Kiernan
> Sent: Thursday, March 16, 2006 11:43 PM
> To: meint002
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Gram's iodine mystery solved
>
> Is this the end of the matter? Who swapped your
> iodine for iron filings? Do you have an enemy
> doing horrid things with the chemicals on your
> shelves?
> --
> -------------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
> kiernan[AT]uwo.ca
> http://publish.uwo.ca/~jkiernan/
> http://instruct.uwo.ca/anatomy/530/index.htm
> _______________________________
> meint002 wrote:
>> Dear Histos,
>>
>> Thanks to all for your advice about making Gram's Iodine soln.
>>
>> There was something wrong about the reagent iodine I was using. I
> borrowed
>> the proper iodine from another histo lab (thanks Luann) and now
>> all is
> just
>> fine. Dissolving it in the Potassium iodide worked like a charm.
>>
>>
>> Joyce Meints
>> Histologist
>>
>> University of Minnesota
>> Paul and Sheila Wellstone Muscular Dystrophy Center
>> MMC 206
>> 420 Delaware St. SE
>> Minneapolis, MN 55455-0392
>>
>> e-mail: meint002@umn.edu
>> lab phone: 612-626-4703
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> The information transmitted is intended only for the person or
> entity to which it is addressed and may contain confidential, and/
> or privileged material. No confidentiality or privilege is waived
> or lost by any errant transmission. If you receive this message in
> error, please immediately delete it and all copies of it from your
> system and notify the sender. E-mail transmission cannot be
> guaranteed to be secure or error-free as information could be
> intercepted, corrupted, lost, destroyed, arrive late or incomplete,
> or contain viruses.
> Barry University - Miami Shores, FL (http://www.barry.edu)
>
>
>
> ------------------------------
>
> Message: 14
> Date: Fri, 17 Mar 2006 09:04:50 -0700
> From: Gayle Callis
> Subject: An aside on viewing kidney biopsies Re: [Histonet]
> microscopes
> To: "Santana, Diane" ,
> Histonet@lists.utsouthwestern.edu
> Message-ID:
> <6.0.0.22.1.20060317090020.01b46438@gemini.msu.montana.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> We supplied laboratories with slides that have the wells in them,
> commonly
> used for viewing larger creatures like insects, etc. These are
> sold by
> Fisher, etc The biospy was floated in PBS, and you can actually use
> any
> microscope at lowest power or stereomicroscope to look for
> glomeruli. This prevented crushing and flattening the biopsy, a
> problem
> we encountered far to many times when bx was examined between a
> slide and
> coverslip immediately after removal.
>
> At 10:55 AM 3/16/2006, you wrote:
>> I am looking at microscopes for kidney bx's. My dept will start
>> assisting
>> with the bx's after the bx has been removed. I have not done this in
>> YEARS... then we watch the tissue, if it floated it was probably
>> fat, if it
>> sank to the bottom of the container it was more than likely
>> tissue. So I am
>> looking for a scope that I can see if I have tissue and some light
>> reading
>> as a refresher course.
>
> Gayle Callis HTL, HT, MT(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Fri, 17 Mar 2006 11:23:54 -0500
> From: "Judith LaDuc"
> Subject: Re: [Histonet] New NY regulations
> To: ,
> Message-ID: <441A9AB5.2A60.00B8.0@trudeauinstitute.org>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi Kathy and NY Histotechicians/technologists,
>
> Yes, the new regulations will affect us. We are classified under the
> heading of clinical laboratory technicians/technologists and are
> subject
> to licensure. I would recommend signing up for the mailing list and
> checking into the website routinely.
>
> The following is a post from the Histonet archives that has a few
> links
> and a timeline of events.
> http://www.histosearch.com/histonet/Jan06/HistonetNYSLicensure.html
> Dr. Kathleen Doyle from NYS will be presenting an informational
> session
> at the Region 1 meeting in Saratoga Springs on Friday, April 28th. The
> Region 1 program (April 28th & 29th) can be accessed at
> http://www.nyhisto.org
>
> Judy LaDuc, BS HTL ASCP
> President, NYS Histotechnological Society
>
>>>> 03/17/06 10:15 am >>>
> Histonetters from New York:
> As stated below will the new regulations affect histotechnicians? Any
> help
> would be appreciated.
> Thanks,
> Kathy Dwyer
>
>
>
> The New York State Office of the Professions has posted updated
> information
> on our state licencing of clinical laboratory technologists,
> technicians and
> Cytotechnologists as pasted below the direct link.
>
> http://www.op.nysed.gov/clp- mar06update.htm
>
> March 2006
> The State Education Department, with the assistance of the State Board
> for
> Clinical Laboratory Technology and interested parties are in the
> process of
> developing draft regulations that will be necessary for the licensure
> of the
> professionals as clinical laboratory technologists, Cytotechnologists,
> and clinical
> laboratory technicians. Once these draft regulations are completed,
> they will
> be submitted to all interested parties and published in the State
> Register
> for comment. At the end of this process, regulations will be presented
> for
> consideration and action by the Board of Regents. It is anticipated
> that this
> process will be completed by mid- summer in 2006. Applications packets
> for licensure
> will not be available until the regulations are enacted. Prior to the
> enactment of the regulations, the Department cannot provide any
> specific information
> to individuals regarding licensure under the "Special Provisions" or
> "grandparenting.." As soon as the regulations are enacted, this
> information will be
> available.
>
> As soon as the regulations are enacted by the State Board of Regents,
> the
> Department will publish a Guide to Practice for the professions of
> Clinical
> Laboratory Technology, Clinical Laboratory Technician, and
> Cytotechnology, as well
> as an application packet for these professions. When they are
> available, the
> applications will be published on this website and can be downloaded
> and
> submitted to the Department. Applicants who wish to receive a hard
> copy
> of the Guide
> to Practice and the Application Packet may submit an e- mail request
> for the
> information now, but should not expect to receive this information
> before the
> summer of 2006. A notice will be put on this website when the
> applications are
> ready to be mailed.
>
> Clinical Laboratory Technicians, Clinical Laboratory Technologists and
>
> Cytotechnologists who want to request to be placed on a mailing list
> now for a hard
> copy of the application packet and receive it in the summer of 2006
> should
> send an e- mail to OPFORMS@mail.nysed.gov, giving their name and
> address, and
> specify the packet they want by:
>
> Code 92A and profession title: Clinical Laboratory
> Technologist/Clinical
> Laboratory Technician, or
> Code 93A and profession title: Cytotechnology.
>
> http://www.op.nysed.gov/clp- mar06update.htm
> Page last updated: 03/15/2006 12:07:36
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Fri, 17 Mar 2006 11:34:15 -0500
> From: "Jacqui Detmar"
> Subject: [Histonet] immunohistochemistry and TUNEL background staining
> To:
> Message-ID:
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi all. I have been doing IHC and TUNELs on mouse placental
> tissues for the past 4 years and have been having problems with
> oodles of background staining during the past 6 months or so. I
> have tried doing avidin-biotin and streptavidin-biotin blocking and
> also using ABC (purchased from Vector) in high pH (TBS, pH 9.4) or
> high salt (TBS [NaCl = 0.5M], pH 7.6) solutions. Nothing seems to
> be working. The IHC using biotinylated secondary antibodies and
> the TUNEL is done using biotinylated dUTP. I have tried using old
> sections that I *know* have worked in the past and I am getting the
> same type of background staining as my newer blocks (i.e. the
> problem is likely not due to fixation (always the same...24 hours
> in 10% phosphate-buffered formalin), nor due to differences in
> paraffin embedding).
>
> So, could my xylene and ethanol solutions be a problem? We get the
> ethanol in-house here at the hospital and the Xylene is purchased
> through Fisher (X16-4). What about differences in TBS or PBS (I
> have tried using both to see if the problem would go away and it
> doesn't make a difference).
>
> Any advice would be appreciated.
>
> Regards,
>
> Jacqui Detmar, Ph.D. student
> Samuel Lunenfeld Research Institute
> Mount Sinai Hospital,
> Toronto, Ontario, Canada
>
>
> ------------------------------
>
> Message: 17
> Date: Fri, 17 Mar 2006 12:03:44 -0500
> From: "John A. Kiernan"
> Subject: Re: [Histonet] Sudan Black Staining
> To: Sharon E Willman
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID: <441AEBF0.58CE6C9C@uwo.ca>
> Content-Type: text/plain; charset=us-ascii
>
> Sudan black B shouldn't bleed. After staining,
> rinse in 70% alcohol until non-lipid structures
> are destained, wash in water, and mount in an
> aqueous medium. The slides are good for months -
> probably longer.
> --
> -------------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
> kiernan[AT]uwo.ca
> http://publish.uwo.ca/~jkiernan/
> http://instruct.uwo.ca/anatomy/530/index.htm
> _______________________________
> Sharon E Willman wrote:
>>
>> Hi,
>> I am working on the Sudan Black stain. Do the slides need to be read
>> immediately due to bleeding of the stain after approximately 24
>> hours?
>>
>> Thanks for any information you can give me about the stain.
>> Sharon Willman
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 28, Issue 28
> ****************************************
>
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