[Histonet] Re: marker for mouse vs. human
If you were using immunofluorescence I could tell you that Hoescht
staining will demostrate punctate staining in AT-rich regions of the mouse
nucleus, whereas human nuclei stain with a smoky appearance. Whether you
could use this same distinction using paraffin sections I don't know. Also
there are human-specific vimentin (DAKO) and Involucrin (BTI) antibodies
available that supposedly will not stain mouse.
Melville B. Vaughan, Ph. D.
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
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