[Histonet] RNALater and cryosectioning
Dear Histonetters,
I have been trying to section RNALater-treated murine pancreas for a
month now and failing miserably. I either get excessive tearing or the
tissue fails to cut entirely. Upon searching, I noticed that several
years ago it was mentioned that sectioning RNALater-treated tissue is
impossible. Has anyone had success with this or is it now general
knowledge that it cannot be done? If you were successful, what did you
do? Thanks in advance for any help.
Kris
--
Postdoctoral Researcher
Tuveson lab
University of Pennsylvania
Abramson Family Cancer Research Institute
421 Curie Blvd.
520 BRBII/III
Philadelphia, PA 19104
Tel: (215)746-7781
Fax: (215)573-2486
Email: kfrese@mail.med.upenn.edu
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