[Histonet] RNALater and cryosectioning

From:Kris Frese

Dear Histonetters,

I have been trying to section RNALater-treated murine pancreas for a 
month now and failing miserably.  I either get excessive tearing or the 
tissue fails to cut entirely.  Upon searching, I noticed that several 
years ago it was mentioned that sectioning RNALater-treated tissue is 
impossible.  Has anyone had success with this or is it now general 
knowledge that it cannot be done?  If you were successful, what did you 
do?  Thanks in advance for any help.



Postdoctoral Researcher
Tuveson lab
University of Pennsylvania
Abramson Family Cancer Research Institute
421 Curie Blvd.
Philadelphia, PA 19104

Tel: (215)746-7781
Fax: (215)573-2486
Email: kfrese@mail.med.upenn.edu

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