[Histonet] Immunofluorscence staining on vibrotome sections
hallo everyone,
I am doing Immunofluorscence staining on vibrotome sections.My problems are:
(1) How to premeablize the tissues? I used 130 um sections from lymph node and embedded in argarose.I tried 0.3% triton or high concentraion of triton x100.But it did not work so well. I also tried with ethanol method.
(2) How can I do antigen retrival on these sections? otherwise I can only use polyclonal or some monoclonal antibodies?
(3) any suggestion about the incubation time ? Normally I incubated on a shaking table at 4°C overnight.
THANK YOU IN ADVANCE
Bin Ma MD
Molecular Biotechnology
German Research Centre of Biotechnology
Mascheroder Weg 1
D-38124 Braunschweig
Germany
Tel: 0049-531-6181293
Fax: 0049-531-6181202
Email: bma@gbf.de
binma2@yahoo.com
SMS schreiben mit WEB.DE FreeMail - einfach, schnell und
kostenguenstig. Jetzt gleich testen! *http://f.web.de/?mc=021192* [http://f.web.de/?mc=021192]
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>