[Histonet] IHC double-labeling -- same secondary?

From:"Anna Elisse Beaudin"

Hi all,

  I am performing double labeling IHC on mouse brain tissue with two
primaries from different species, one raised in rat, the other in goat. 
I was wondering if it was kosher (or even preferred) to use both a
donkey anti-rat secondary and a donkey-anti goat secondary.  if so, can
I incubate with both together as a cocktail?  my donkey anti-rat is
flourophore conjugated, and I was planning to use biotinylated donkey
anti-goat followed with a flourophore-conjugated streptavidin.  any
suggestions/comments would be greatly appreciated.

Thanks in advance,
Anna Beaudin
Division of Nutritional Sciences
Cornell University

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