[Histonet] IHC double-labeling -- same secondary?
|From:||"Anna Elisse Beaudin" |
I am performing double labeling IHC on mouse brain tissue with two
primaries from different species, one raised in rat, the other in goat.
I was wondering if it was kosher (or even preferred) to use both a
donkey anti-rat secondary and a donkey-anti goat secondary. if so, can
I incubate with both together as a cocktail? my donkey anti-rat is
flourophore conjugated, and I was planning to use biotinylated donkey
anti-goat followed with a flourophore-conjugated streptavidin. any
suggestions/comments would be greatly appreciated.
Thanks in advance,
Division of Nutritional Sciences
Histonet mailing list
<< Previous Message | Next Message >>