[Histonet] Frozen embedding of lungs

From:Kim O'Sullivan

Hi all,

Does any one out there have a successful method for inflating mouse lungs to freeze. We currently freeze them unfixed in OCT, but of course the morphology is shocking as the lungs have collapsed. Is there a method that preserves the morphololgy without collapsing or imploding the lungs.

Any advice will be appreciated

Kim O'Sullivan
Centre for Inflammatory Diseases
Department of Medicine
Monash University

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