[Histonet] Frozen embedding of lungs
Hi all,
Does any one out there have a successful method for inflating mouse lungs to freeze. We currently freeze them unfixed in OCT, but of course the morphology is shocking as the lungs have collapsed. Is there a method that preserves the morphololgy without collapsing or imploding the lungs.
Any advice will be appreciated
Kim O'Sullivan
Centre for Inflammatory Diseases
Department of Medicine
Monash University
Melbourne
Australia
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