[Histonet] Double stain ?

From:"Emerson, Rachael"

Hello. I am attempting to do a sequential double stain with 2 different
Unfortunately, one requires using Triton-X 100 for antigen retrieval and the
other requires using trypsin.
I've run trials using several different antigen retrieval methods and these
are the only ones that work=2E
I'm afraid that performing 2 different retrieval steps will cause the stain
not to work. I would really appreciate any thoughts or suggestions.
I am working with paraformaldehyde fixed mouse embryos.

Rachael Emerson

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