Hi everyone,
   
  A client is interested in purchasing a used H&E x-y stainer as well as looking into both used and new IHC stainers.  Any information you have would be greatly appreciated.
   
  Louri Roberts
  
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Today's Topics:

1. RE: CAP Immuno Survey Slides (Richard Cartun)
2. RE: CAP Immuno Survey Slides (Dana Settembre)
3. Aluminium localization (Lina Vieira)
4. RE: CAP Immuno Survey Slides (Dawson, Glen)
5. Re: Health and Safety Guidelines for the Lab by L Montgomery
needed (jason.burrill@crl.com)
6. eosin Y (Jennifer MacDonald)
7. RE: Aluminium localization (Monfils, Paul)
8. Good used microtome for sale? (MVaughan4@ucok.edu)
9. multi tissue block for keratin (Nava, Josefa)
10. Marker for mouse vs human tissue (James Watson)
11. paraffin tissue embedding and cutting (karen Cai)
12. air filling Re: [Histonet] Frozen embedding of lungs
(Gayle Callis)
13. Re: insect histology book inquiry (Gayle Callis)
14. Help needed!!! Immunofluorescence double staining ofwith
mouse lung (Chengming Wang)
15. RE: paraffin tissue embedding and cutting (Kemlo Rogerson)
16. RE: Breast core biopsies, same day results by frozen s
ections (Kemlo Rogerson)
17. Disposal of specimens/formalin (Vicki Gauch)
18. DAB (Susan.Ferrigon@sanofi-aventis.com)
19. F4/ 80 (Susan.Ferrigon@sanofi-aventis.com)
20. RE: RE: Double staining (Guillermo Palao)
21. Re: Disposal of specimens/formalin (Gayle Callis)
22. job opening: cool location--with a nice addition of temp to
perm. (Cheryl)
23. Re: Disposal of specimens/formalin (tracy.bergeron@crl.com)
24. microwave technology advance (Edwards, R.E.)


----------------------------------------------------------------------

Message: 1
Date: Thu, 30 Mar 2006 12:59:26 -0500
From: "Richard Cartun" 
Subject: RE: [Histonet] CAP Immuno Survey Slides
To: "JUAN GUTIERREZ" , "Histonet
(E-mail)" , "Laura Jones"

Message-ID: 
Content-Type: text/plain; charset=US-ASCII

I agree. I will forward this to a member of the CAP Cell Markers
Committee.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax

>>> "GUTIERREZ, JUAN" 03/30/06
12:44PM >>>
We pulled the labels off. What a pain in the .... To: CAP, please
don't do that again.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer. Long
live free speech!


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Thursday, March 30, 2006 11:05 AM
To: Histonet (E-mail)
Subject: [Histonet] CAP Immuno Survey Slides

Hello Histoland. We were wondering if anyone else who may have
participated
in the recent CAP survey had experienced any problems with the labels
on
the
slides. We performed some of the survey by hand (our machine is
down!)
using the Shandon Sequenza coverplates, and it seemed like the label
adhesive was blocking the reagents from passing onto the slide. We
then
repeated some of the slides on a demo machine, after verifying our
antibodies, and even then it seemed like that adhesive was interfering
with
the reagents. We also lost any info we had written on the slide
labels,
even with the impervious markers, during automated processing. 

We didn't remember our survey slides ever having labels before, and
are
just
curious if anyone else had encountered this. Thanks in advance! 

The Histochicks at Sharon Regional, Sharon, PA

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet







------------------------------

Message: 2
Date: Thu, 30 Mar 2006 13:39:30 -0500
From: "Dana Settembre" 
Subject: RE: [Histonet] CAP Immuno Survey Slides
To: "JUAN GUTIERREZ" , "Richard
Cartun" , "Histonet (E-mail)"
, "Laura Jones"

Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Please do not send us slides with labels.
They were really difficult to work with.

Dana Settembre
University Hospital - UMDNJ
Newark, NJ

>>> Richard Cartun 03/30/06 12:59 PM >>>
I agree. I will forward this to a member of the CAP Cell Markers
Committee.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax

>>> "GUTIERREZ, JUAN" 03/30/06
12:44PM >>>
We pulled the labels off. What a pain in the .... To: CAP, please
don't do that again.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer. Long
live free speech!


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Thursday, March 30, 2006 11:05 AM
To: Histonet (E-mail)
Subject: [Histonet] CAP Immuno Survey Slides

Hello Histoland. We were wondering if anyone else who may have
participated
in the recent CAP survey had experienced any problems with the labels
on
the
slides. We performed some of the survey by hand (our machine is
down!)
using the Shandon Sequenza coverplates, and it seemed like the label
adhesive was blocking the reagents from passing onto the slide. We
then
repeated some of the slides on a demo machine, after verifying our
antibodies, and even then it seemed like that adhesive was interfering
with
the reagents. We also lost any info we had written on the slide
labels,
even with the impervious markers, during automated processing. 

We didn't remember our survey slides ever having labels before, and
are
just
curious if anyone else had encountered this. Thanks in advance! 

The Histochicks at Sharon Regional, Sharon, PA

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 3
Date: Thu, 30 Mar 2006 20:11:06 +0100
From: "Lina Vieira" 

Subject: [Histonet] Aluminium localization
To: "Histonet" 
Message-ID: <007301c6542d$b28574f0$2914100a@labhistologia>
Content-Type: text/plain; charset="iso-8859-1"

In return to the Aluminium localization, 
I would now if an excessive time of fixation (in formaldeide), or an excessive time of etanol 70% imersion (for example 4 days)can induce false negative results to the aluminum? 


Thanks in advance for any information you can provide,

Lina Vieira
University of Algarve
Portugal

_______________________________________________

------------------------------

Message: 4
Date: Thu, 30 Mar 2006 13:14:47 -0600
From: "Dawson, Glen" 
Subject: RE: [Histonet] CAP Immuno Survey Slides
To: "Histonet \(E-mail\)" 
Message-ID:

Content-Type: text/plain; charset="iso-8859-1"

All,

I've talked to the CAP about this issue and they said that they would "take it under advisement". Just goes to show you that those in charge of sending out these CAP surveys for IHC have no experience in the actual staining of IHC slides.

Also, the labels they put on are heavy duty and the adhesive they leave behind is NOT condusive to automated coverslipping (with the Sakura Glass coverslipper). They tend to fall off the suction cup and get broken by the conveyor. I would strongly suggest coverslipping these slides by hand until the CAP decides to stop this craziness.

Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dana
Settembre
Sent: Thursday, March 30, 2006 12:40 PM
To: JUAN GUTIERREZ; Richard Cartun; Histonet (E-mail); Laura Jones
Subject: RE: [Histonet] CAP Immuno Survey Slides


Please do not send us slides with labels.
They were really difficult to work with.

Dana Settembre
University Hospital - UMDNJ
Newark, NJ

>>> Richard Cartun 03/30/06 12:59 PM >>>
I agree. I will forward this to a member of the CAP Cell Markers
Committee.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax

>>> "GUTIERREZ, JUAN" 03/30/06
12:44PM >>>
We pulled the labels off. What a pain in the .... To: CAP, please
don't do that again.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer. Long
live free speech!


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu 
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Thursday, March 30, 2006 11:05 AM
To: Histonet (E-mail)
Subject: [Histonet] CAP Immuno Survey Slides

Hello Histoland. We were wondering if anyone else who may have
participated
in the recent CAP survey had experienced any problems with the labels
on
the
slides. We performed some of the survey by hand (our machine is
down!)
using the Shandon Sequenza coverplates, and it seemed like the label
adhesive was blocking the reagents from passing onto the slide. We
then
repeated some of the slides on a demo machine, after verifying our
antibodies, and even then it seemed like that adhesive was interfering
with
the reagents. We also lost any info we had written on the slide
labels,
even with the impervious markers, during automated processing. 

We didn't remember our survey slides ever having labels before, and
are
just
curious if anyone else had encountered this. Thanks in advance! 

The Histochicks at Sharon Regional, Sharon, PA

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 5
Date: Thu, 30 Mar 2006 14:22:19 -0500
From: jason.burrill@crl.com
Subject: [Histonet] Re: Health and Safety Guidelines for the Lab by L
Montgomery needed
To: histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset="US-ASCII"

Thanks to all that responded and all your offers. Thanks to Victor Tobias 
I found a used copy at http://www.abebooks.com. It is a very difficult 
book to get your hands on.
Jason



Jason D. Burrill
Manager, Histology 
Charles River Laboratories
251 Ballardvale Street
Wilmington, MA 01887
ph: 978-658-6000 ext 1652
fax: 978-988-8793
E-mail: jason.burrill@crl.com

------------------------------

Message: 6
Date: Thu, 30 Mar 2006 11:25:29 -0800
From: Jennifer MacDonald 
Subject: [Histonet] eosin Y
To: Melanie Black 
Cc: histonet@lists.utsouthwestern.edu,
histonet-bounces@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset="US-ASCII"

What is the pH of the eosin?







Melanie Black 
Sent by: histonet-bounces@lists.utsouthwestern.edu
03/30/2006 03:45 AM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] GMA & Humidity






Dear All,
I would like to ask about a particular problem with eosin-Y staining. I am
using Eosin Y alcoholic without phloxine (from Sigma) and for some reason,
all of the eosin staining comes out of the tissue as it is being
dehydrated. I can actually see that it just drains out. I know that this 
is
part of the differentiation process but it just all comes out. We use the
same staining set for mouse liver tumour tissues with no problem. These 
are
routinely processed (by a hospital pathology lab) rat rectum slides. Is
the phloxine important for "keeping in the stain"? The sections are quite
small. Should I be using a different eosin?
Any advice on this matter would be greatly appreciated.
Cathy



Cathy

JB 4 resin is one of the few resins that is water soluble, and 
complete dehydration of tissue is NOT required. In the processing of 
this resin, clearing agents like xylene & chloroform are unnecessary. 
I have used this resin many times, specially for tissue containing 
gels etc.

Regards
Melanie.
-- 
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
Chris Barnard Building
University of Cape Town
Anzio Road
Observatory
7925
Republic of South Africa

Tel +27 21 406-6589
Cel +27 82 469-3352
Fax +27 21 448-5935

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Thu, 30 Mar 2006 14:45:14 -0500
From: "Monfils, Paul" 

Subject: RE: [Histonet] Aluminium localization
To: "'histonet@lists.utsouthwestern.edu'"

Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE017176B6@lsexch.lsmaster.lifespan.org>

Content-Type: text/plain; charset="iso-8859-1"

Storage of tissue in alcohol won't affect the presence or reactivity of
aluminum. Formalin should not have a deleterious effect either, if it is
properly buffered. I believe long-term storage in acidic formalin (or any
acidic solution) might dissolve aluminum out of tissue, though I don't have
any reference or direct experience to corroborate this.

> ----------
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lina
> Vieira
> Sent: Thursday, March 30, 2006 11:11 AM
> To: Histonet
> Subject: [Histonet] Aluminium localization
> 
> In return to the Aluminium localization, 
> I would now if an excessive time of fixation (in formaldeide), or an
> excessive time of etanol 70% imersion (for example 4 days)can induce false
> negative results to the aluminum? 
> 
> 
> Thanks in advance for any information you can provide,
> 
> Lina Vieira
> University of Algarve
> Portugal
> 
> _______________________________________________
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 



------------------------------

Message: 8
Date: Thu, 30 Mar 2006 14:19:37 -0600
From: MVaughan4@ucok.edu
Subject: [Histonet] Good used microtome for sale?
To: histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset="US-ASCII"

Histonetters,
I might be looking for a good used microtome in the next few months 
(provided the grant comes in). I have a good working AO820 from the 60's? 
but it has a lot of trouble sectioning anything less than 8 microns thick, 
and I don't have one of those newer disposable blade holders either. I 
will be glad to pay a reasonable price for it. Thanks.
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm

------------------------------

Message: 9
Date: Thu, 30 Mar 2006 14:47:20 -0600
From: "Nava, Josefa" 
Subject: [Histonet] multi tissue block for keratin
To: 
Message-ID:
<2C515C1049EAF5459EFD8C9B929078A419463E@phdex03.txhealth.org>
Content-Type: text/plain; charset="us-ascii"

Hello Everyone,

I need some suggestions please on what kind of tissues do I need to
put together to build a multi control tissue for different
subtypes of Keratin? Any additional information you can provide me is
greatly appreciated on how to go about creating a multi tissue control
block. Thank you so much.

Josie Nava


The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system.

------------------------------

Message: 10
Date: Thu, 30 Mar 2006 12:51:12 -0800
From: "James Watson" 
Subject: [Histonet] Marker for mouse vs human tissue
To: 
Message-ID: 
Content-Type: text/plain; charset="us-ascii"

I am looking for an antibody to differentiate human cells from mouse
tissue in human implants in mouse. I have tried to locate a human
mitochondrial marker, but have only been able to find it made in mouse.
Any information would be appreciate. Thank you.

James Watson HT, ASCP
Facilities Manager of Histology
GNF, Genomics Institute of the Novartis Research Foundation Room C015
858-332-4647 jwatson@gnf.org 



------------------------------

Message: 11
Date: Thu, 30 Mar 2006 14:13:21 -0800
From: "karen Cai" 
Subject: [Histonet] paraffin tissue embedding and cutting 
To: 
Message-ID: <000e01c65447$288b0b10$7d01a8c0@prosci.com>
Content-Type: text/plain; charset="windows-1250"

Hello there,
I have some questions regarding embedding and cutting paraffin tissue
block:

1. After embedding, I found there are a lot of bubbles inside the
paraffin tissue block (I put the mold at RT). Sometimes it has,
sometimes not. I don’t know why. How to avoid this?
2. Sometimes I found if I put the paraffin tissue sections into
the 37C water bath, the paraffin will be diffused very quickly. Also the
color of the whole paraffin tissue block look strange: white, not clear
white. I am jus wondering whether the method of embedding tissue is
wrong, or something else?

Any kind of help will be very appreciated,

Thanks in advance,

Best,
Karen




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------------------------------

Message: 12
Date: Thu, 30 Mar 2006 15:51:19 -0700
From: Gayle Callis 

=== message truncated ===

		
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