Invitrogen (formerly Caltag) has a B-cell marker that works great on mouse tissue it is CD45r (B220) both purified and biotin labelled.  I used it on our new Bond Max autostainer from Vision Bio Sytems and had excellant results with FFPE tissue.

-Tom Wood
Michigan State University
Diagnostic Center for Population and Animal Health


>>> "Marilyn Johnson"  03/18/06 07:17PM >>>
Hi Histonetters,
In approximately two months, our Lab. will be initiating a new project using skin sections of mice.
We will be using IHC to demonstrate infiltrates at vaccine sites including CD4, CD8, B-cells, 
monocytes, macrophages, etc. 
Any assistance would be greatly appreciated.
Thank you in advance. 

Marilyn Johnson
Alberta Agriculture
Edmonton, Alberta, Canada
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