I totally agree with Andrea.  Another superb two part DAB substrate kit is 
Pierce DAB (brown) $65 for 250 ml kit or Pierce CN/DAB (black) $78 for 250 
ml kit, and you can make up as much or little as you want for number of 
slides in a run.

Acadamic labs often get discounted pricing and free shipping other than 
hazardous material costs, this can be purchased through Fisher and probably 
VWR too.

At 01:41 PM 3/22/2006, you wrote:
>I too am in academic labs so relate to the cost concern; however, I 
>strongly recommend DAB in liquid kit form - either DAB+ from DAKO or from 
>Zymed. They are liquid ready to use concentrates diluted into buffer 
>either supplied with the kit or you provide yourself.
>
>I have used both for years and years now with excellent results. Worth the 
>extra bucks (about 80-100 dollars for 110 mls which is actually quite a 
>lot) for consistent results time after time after time - without the 
>hazards of working with powder or solids.
>
>Good luck!
>Andrea

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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