There are several alternatives for negative controls, but a common one is
simply to use the buffer rinse in place of the primary antibody, thereby
removing the first link in the chain. Another way is to keep everything the
same and run the IHC stain on negative tissue (no antigen present in the
tissue).

----- Original Message ----- 
From: "cynthia haynes" 
To: 
Sent: Wednesday, March 08, 2006 6:49 AM
Subject: [Histonet] negative immunohistochemistry control


> Good Morning everyone, I have a question about immuno
> staining. I've been away from this type of staining
> for a while and now I am doing them again on a regular
> basis. Why do you run a negative control with each
> run? Are you only suppose to put the normal serum on
> negative control only? I've forgotten; would someone
> please answer these questions for me. Thanks in
> advance.
>
> Cynthia Haynes H.T.
>
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> Histonet@lists.utsouthwestern.edu
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