Re: [Histonet] histochemistry- alanine aminotransferase -rat liver

From:John Kiernan

This enzyme is mentioned in a few places in Volume 
3 of Stoward & Pearse's Histochemistry (3rd edn, 
1991). A general method for aminotransferases is 
given on p.583. It is also necessary to read the 
explanatory material about the technique on pp 
173-176 and elsewhere. This is a rather involved 
technique, using two auxiliary enzymes, one of 
which may not be needed.

A Google Scholar search for
"alanine aminotransferase" histochem*
brings up 405 references. The ones at the top of 
the heap are papers in J. Histochem. Cytochem. in 
the 1980s. The full text of these papers (with 
illustrations) will be available as .pdf files, 
free for anyone in the world to download, at the 
journal's web site:

Your email to Histonet (cited below) refers to lab 
   safety, standardization and other buzz-words. 
Are you asking how to do the histochemical method, 
or how to make safety regulations? If you are a 
researcher, you need to consult the books and 
internet resources mentioned above. If you are a 
safety officer, be assured that aminotransferase 
histochemistry involves no exposure to hazardous 
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
_______________________________ wrote:
> Dear all,
>                  This is Dr.Kamalavenkatesh from wockhardt reserch
> center,India. As a part of pre clinical safety evaluation of our NCE'S We
> want to standardize the methodology for quantitative histochemistry of
> Alanine aminotransfrease(ALT) activity in rat liver that will aid in direct
> correlation between histopathological findings to that of histochemical
> findings.It seems that literature regarding the methodology is
> scanty.Hence, I want to know from you that any body is doing this technique
> in their laboratory, if so the protocol involved and their experience with
> the method. Please let us know.
> Thanks and regards
> kamalavenkatesh
> Please Visit our New Corporate Web Site
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John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

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