Re: [Histonet] Mayer's Hematoxylin
Karen,
Our students are using Hematoxylin 1, by Richard= -Allan. They are
staining by hand, so there is great variation of tec= hniques between
students, but very little in the staining of the nuclei.&nb= sp;
Richard-Allan has a great chart for optimizing the H&E with their r eagents.
Jennifer MacDonald
-----histonet-bounces@lists.utsouthweste= rn.edu wrote: -----
To:
From: "Bauer,= Karen"
Sent by: histonet-bounces@lists.uts= outhwestern.edu
Date: 03/15/2006 09:02AM
Subject: [Histonet] Mayer's = Hematoxylin
Hello to all,
I'm looking for a Pre-Made Mayer's H= ematoxylin that we can use
for our routine H&E staining procedure. &nbs= p;We make up our
Mayer's Hematoxylin from scratch, but have recently been g= iven
permission to buy something that is pre-made. We've tried Harris , but the doctors do not like the differentiation that take place
with usin= g that kind of hematoxylin. (We stain by hand, so one
tech's dip migh= t be longer or shorter than another tech's dip in
the differentiator.) &nbs= p;They are seeing variations in nuclear
staining, no matter how careful we = are with our "dips".
I'm looking for something that stains ti= ssues in 5-10 minutes and
does not need differentiation. Everything t= hat I've tried so far
is not working out to their satisfaction. We us= e a Gill's III
for our FS set up, and they do not want that either (for the routine H&E's).
Does anyone have any ideas or tips for me? &nbs= p;I'll be greatly
appreciative.
Thanks in advance,
Karen Baue= r HT(ASCP)
Histology Supervisor
Luther Hospital
Eau Claire, = WI
********************Confidentiality Notice********************
This message is intended for the sole use of the individual and en tity to whom it is addressed, and may contain information that is
privilege= d, confidential and exempt from disclosure under
applicable law. Any unauth= orized review, use, disclosure or
distribution of this email message, inclu= ding any attachment, is
prohibited. If you are not the intended recipient, = please advise
the sender by reply email and destroy all copies of the origi= nal
message. Thank you.
______________ ______________________ 5F__= ________
Histonet mailing list
Histonet@lists.utsouth= western.edu
[1]http://lists.utsouthwestern.edu/mailman/listinfo= /histonet
References
1. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>