Re: [Histonet] Aqueous mountant for fluorescence work

From:Gayle Callis

Molecular Probes Prolong Gold antifade ready to use, a hard set.  You still 
need to seal it but do this with diluted permanent mounting media (use the 
same solvent contained in the mounting media).  If you do NOT seal it, it 
will retract over time.  Fingernail polish is not ideal if GFP is involved, 
it can cause the GFP to lose fluorescence.

Vector Laboratories makes a hard set, Vectashield Hard Set.

Both companies also have these mounting medias with DAPI if you wish to 
have your nuclei stained.

At 09:30 AM 3/14/2006, you wrote:
>Dear Histonet,
>Does anyone know of an aqueous mounting medium which doesn't
>fade fluorescently-labelled cell preparations and which sets hard for
>convenient storage?
>Ms.Orla Gallagher
>Academic Unit of Bone Biology
>Division of Clinical Sciences (South)
>D Floor (DU20) Medical School
>Henry Wellcome Laboratories for Medical Research
>University of Sheffield
>Beech Hill Road
>Sheffield S10 2RX
>Tel:   0114 271 3783(lab)
>        0114 271 3954(office)
>Fax:   0114 271 1711
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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