Diana

We used dako's ph9 retreival in the pascel with good results and
envision rabbit as a detection system.  We were staining human thymus
grafts in mouse tissue.  30 minute incubation at 1:100.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana
McCaig
Sent: Wednesday, March 15, 2006 9:33 AM
To: Histonet (E-mail)
Subject: [Histonet] T Cell Heat retrieval using microwave pressure
cooker



Hi
I am trying to demonstrate T cells using Polyclonal Rabbit Anti human
CD3, T cell (DAKO ENVISION).  The control tissue is tonsil and using a
manual procedure.  I have increased the time in the microwave pressure
cooker from 5 up to 30 minutes (pH 6 buffer) and have no success.  The
antibody is incubated for 30 minutes (recommended) but I have gone as
high as 1 hour.  I am using AEC and Mayers counterstain.  Any help or
advise would be greatly appreciated.
				Diana 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


__________ NOD32 1.1444 (20060315) Information __________

This message was checked by NOD32 antivirus system. http://www.eset.com



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet