Are you hand staining or using an automated stainer?  Back when I did BM
smears, we found that they had to be run through the stainer 2 or 3 times
due to the thickness of the smear.  This did work well however; and we
didn't have any problems with nuclear staining or "over staining".

~ Ford

Ford M. Royer, MT(ASCP)
Minnesota Medical Specialists
Minneapolis, MN

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ravishankar
Nagarajan
Sent: Tuesday, March 07, 2006 9:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 11




Dear Group,
  Can anybody provide me the procedure for Wright Giemsa staining procedure
for Bone marrow smears. We are performing the Wright's procedure here and we
get inconsistent results here. Like the nuclei do not get stained. Could it
be due to the fact that Wright's is only a cytoplasmic stain. Any
suggestions would be welcome. I also include the procedure by which we do
Wright's here.

Wright's stain - 8 - 10 minutes
Sorensen's buffer  (pH - 7) - 8 -10 minutes

Regards and Thanks,
Dr.N>Ravishankar

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