Thanks a lot to everybody, it seems Lab Vision´s Ab is the most used. According to manufacturer it is optimized to work in paraphin-embedded samples. Does anybody have experience with frozen sections?
   
  Thank you all for your useful tips!
   
  Guillermo

pruegg@ihctech.net escribió: 
  I would recommend Rabbit Monoclonal Ki67 from Lab Vision, I have used it on
mouse tissues with good results.
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Trajkovic,
Dusko
Sent: Friday, March 03, 2006 11:13 AM
To: Guillermo Palao; Histonet
Subject: RE: [Histonet] Ki67 on mouse kidney tissue

I also use a Rabbit Monoclonal Ki-67 from Lab Vision. Works great.
On Ventana Discovery XT: DAB Map Kit / CC1 Standard / Primary at 1:800 / GaR
(Dako) 1:200.
Dusko trajkovic

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo
Palao
Sent: Friday, March 03, 2006 9:05 AM
To: Histonet
Subject: [Histonet] Ki67 on mouse kidney tissue

Hello all,

Does anybody recommend a particular rabbit anti-Ki67 Ab that works well
in mouse kidney? I have seen many different commercial Abs but I would like
some first-hand information before buying one.

Thanks in advance,


Guillermo Palao, MD. Ph.D.
Laboratorio de Reumatología
Centro de Investigación
Hospital 12 de Octubre
Avda de Córdoba s/n
Madrid 28041
Spain

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