RE: [Histonet] Combining Hema 3 and immuno detection

From:"Elizabeth Chlipala"


We have had good success in combining IHC with the chromagen DAB and a
wide variety of special stains.  There is a nice article in Histologic,
I'm not sure what issue that covers IHC and PAS staining.  There are
some pictures on our web page that shows DAB IHC in combination with
Acid Fast.  We run the immuno first and then use whatever special stain
we need as the counterstain.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
[] On Behalf Of Ashley
Sent: Wednesday, March 08, 2006 3:43 PM
Subject: [Histonet] Combining Hema 3 and immuno detection

Hi All,


Can anyone recommend the best way to combine immunodetection (either via
fluorescence or enzyme) and a differential stain such as Hema 3 (or
another Wright Giemsa like stain)?  I'm trying this with cytospins and
have had poor luck.  So far either the cell morphology is bad and the
signal is good or the signal is nonexistent but the cell morphology is


Thanks in advance!


Ashley N. Haines, PhD.

Postdoctoral Research Associate

Virginia Institute of Marine Science

Department of Environmental & Aquatic Animal Health

PO Box 1346 

Gloucester Point, VA 23062



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