[Histonet] organotypic hippocampal cultures and immunohistochemistry
From: | =?iso-8859-2?Q?Jakub_Ot=E1hal?= |
Hi Histoneters,
I have problem with immunohistochemistry on organotypic hippocampal slice
cultures (OHSC). I have stained them with OX-42(CD11b) and
glucoseoxidase-DAB development but the staining is weak and unsharp. When I
tried the same protocol on rat brain slices the staining is very nice. OHSC
are fixed for 1hour in 4% PFA then 20%sucroze and then glued on freezing
stage and cut with microtome to 30mikron slices. Does anybody have an idea
where is the bug?
Thank you very much
Best regards
jakub
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Jakub Otahal MD,PhD
Department of Developemental Epileptology
Institute of Physiology
Czech Academy of Sciences
Videnska 1083
142 20 Prague 4
Czech Republic
tel: +420 241062495
jotahal@ftvs.cuni.cz
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