[Histonet] how to unfold section after mounting?
Does anyone have experience trying to unfold frozen (30 micron) section after IF staining and mounting (dried overnight, mounted in dpx after dehydration)? My guess is once it binds it is bound forever. A small piece with important and nice staining folded on itself, it could be seen only after high mag. Wondering if the section could be somehow separated from fisher brand superfrost plus slide by light steaming or with excess of Triton or else to remount. would appreciate ideas or practical advice,
Dr. Igor O. Nasonkin Ph.D.
Johns Hopkins University
Ross Research Bldg Rm 563
720 Rutland Ave
Baltimore MD 21205
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