[Histonet] dehydration->xylene->DPX for immunofluorescence? what is your opinion?
What is your practical experience with using dehydration->xylene->DPX mounting of fluorescently labeled sections?
My graduate lab used only aqueous medium for coverslipping fluorescently labeled sections. I always heard alcohol-xylene is a no-no for fluorescence, even here (my postdoc.lab). However my PI insists on using alcohol dehydration and mounting in xylene-based DPX because "xylene removes lipids from brain sections and makes the signal more crisp.
I tried both, and both (aqueous and xylene-based methods) work and produce nice fluorescent signal. Is there a general guideline here which method is technically correct?
Thans for your response,
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