Dear histonetters,

What is your practical experience with using dehydration->xylene->DPX mounting of fluorescently labeled sections?

My graduate lab used only aqueous medium for coverslipping fluorescently labeled sections.  I always heard alcohol-xylene is a no-no for fluorescence, even here (my postdoc.lab). However my PI insists on using alcohol dehydration and mounting in xylene-based DPX because "xylene removes lipids from brain sections and makes the signal more crisp.
I tried both, and both (aqueous and xylene-based methods) work and produce nice fluorescent signal. Is there a general guideline here which method is technically correct?

Thans for your response,
Igor



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