Dear Group,
  Can anybody provide me the procedure for Wright Giemsa staining procedure for Bone marrow smears. We are performing the Wright's procedure here and we get inconsistent results here. Like the nuclei do not get stained. Could it be due to the fact that Wright's is only a cytoplasmic stain. Any suggestions would be welcome. I also include the procedure by which we do Wright's here.

Wright's stain - 8 - 10 minutes
Sorensen's buffer  (pH - 7) - 8 -10 minutes

Regards and Thanks,
Dr.N>Ravishankar

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