[Histonet] Question

From:"DIXON Felicia"


I am having major problems sectioning human hair that is embed in
nitrocellulose and paraffin.  I have tried cutting with a glass knife and a
low profile blade.  I am working from a literature method but am new to
embedding and mictrotomy.  In addition to problems with cutting uniform
cross-sections, I am losing a lot of fibers after clearing with xylenes and
then oxidizing with performic acid.  If anybody could give me suggestions on
the best way to embed and cut hair then how to keep the cross-sections on
the slide for treatment, I would greatly appreciate it.

Felicia Dixon


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