[Histonet] Negative IHC control

From:Patti Loykasek

Just a few more thoughts on the negative control issue. Everyone has raised
very good & valid points. For the CAP checklist, it is good to remember the
intent behind the question.
I think a vital piece of information, is to know your IHC 'system' -
antibodies, detection system, pretreatment, etc... This involves both the
pathologist and the technologist. Both should have enough knowledge &
experience to determine specificity & sensitivity of all of the components
of the system. If you are not familiar with your antibodies & how they look,
all the possible negative controls wonıt help much. It is important when
bringing Œon-lineı new antibodies to test them on many tissues- both tumor &
normal that should show positivity & negativity. Determine what the
specificity & sensitivity is in your lab. There are varying pre-analytical
factors that can contribute to non-specific staining. If you are very
familiar with your Œsystemı, than running multiple negative controls is not
necessary. In the clinical setting, it is important not to waste a patientıs
precious diagnostic biopsy by running every possible permutation of negative
control ­ I have seen this happen on more than one occasion.
Just my thoughts on the issue.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

This e-mail message, including any attachments, is for the sole use of
the intended recipients and may contain privileged information. Any 
unauthorized review, use, disclosure or distribution is prohibited. If 
you are not the intended recipient, please contact the sender by e-mail 
and destroy all copies of the original message, or you may call PhenoPath 
Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
Histonet mailing list

<< Previous Message | Next Message >>